《植物生理学报》 2012, 48(3): 265-271

H₂O₂作为根源信号介导盐胁迫诱导的蚕豆气孔关闭反应

安国勇, 李保珠, 武桂丽, 宋纯鹏*

棉花生物学国家重点实验室, 河南省植物逆境生物学重点实验室, 河南大学生命科学学院, 河南开封475004

通信作者：宋纯鹏；E-mail: songcp@henu.edu.cn

摘　要：

H2O2作为信号分子可被多种胁迫诱导产生并在细胞内积累, 进而参与调节植物的抗逆反应。文章通过远红外热成像观察等实验发现, 根部NaCl胁迫可诱导蚕豆气孔关闭, 叶片温度上升, 叶片内Na+和H2O2含量增加, 蒸腾流汁液中H2O2浓度升高。另外, NaCl可直接诱导离体蚕豆根产生H2O2, 却不能影响表皮条内H2O2含量。NaCl胁迫条件下产生的蒸腾流汁液可直接诱导表皮条气孔关闭, 该过程可被抗氧化剂抗坏血酸(AsA)所逆转。这些结果表明, H2O2作为盐胁迫的根源信号, 可能通过维管系统运输参与调节蚕豆气孔的关闭反应。

关键词：盐胁迫; 过氧化氢; 气孔关闭; 根源信号

收稿：2011-11-01   修定：2012-02-01

资助：国家自然科学基金(31071240和31140071)、教育部科学技术研究重点项目和省部共建河南大学科研项目。

H₂O₂ Could Act as Root Source Signal to Mediate Stomatal Closure Induced by Salt Stress of Vicia faba L.

AN Guo-Yong, LI Bao-Zhu, WU Gui-Li, SONG Chun-Peng*

State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China

Corresponding author: SONG Chun-Peng; E-mail: songcp@henu.edu.cn

Abstract:

As signaling molecules, hydrogen peroxide (H₂O₂) generation is induced in plants following exposure to a wide variety of biotic and abiotic stimuli. In this paper, we found that high NaCl concentration in rhizosphere could induce leaf temperature increasing and stomata closing, and increase the concentrations of Na₊ and H₂O₂ in Vicia faba leaves. The transpiration stream under NaCl stress could induce the stomata closuring, and ascorbic acid (AsA) could partly abolish the transpiration stream-induced stomatal closure. Further experiment indicated that NaCl could not directly induce H₂O₂ production and stomatal closure in detached leaves, but could directly induce H₂O₂ production in detached roots. Those indicate that H₂O₂ may act as root source signal transported by fibrovascular system and mediate stomatal closure of V. faba.

Key words: salt stress; hydrogen peroxide (H₂O₂); stomatal closure; root source signal